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अमूर्त

Use of Nitrifying Bacteria for Promoting Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Nursery Phase in Indoor System

Suantika G*, Turendro OR and Situmorang ML

This research aims to determine the effect of periphytic biofilter in static culture of giant freshwater prawn Macrobrachium rosenbergii de Man through the application of nitrifying bacteria in 50 l containers equipped with CaCO3 as substrate for the nitrifying bacteria and bamboo shelter as a substrate for the periphytic attachment. This experiment was carried out by following three consecutive stages: (1) inoculation of nitrifying bacteria into the culture at the concentration of 105 CFU.mL-1, 24 h before the prawn Post Larvae age 40 days (PL40) was stocked; (2) stocking of prawn PL40 into four treatment groups with different number of bamboo shelter: without shelter (0) at the prawn density of 10 individuals per container, and with addition of 2, 4, or 8 shelters, at the prawn density of 30 individuals per container; and (3) measurement of biological, physical, chemical and microbiological parameters of water quality. Following 60 days culture period, optimum performance was obtained in the culture with 4 shelters with the prawn final body weight, length, and total biomass of 1.00 ± 0.04 g, 4.91 ± 0.03 cm, and 63.17 ± 4.27 g, respectively. The observed water quality parameters (level of ammonium, nitrite, nitrate, dissolved oxygen, pH and temperature) were within the tolerance level for prawns and were relatively stable during the culture period. Moreover, a stable microflora community was observed, with the abundance of periphytic bacteria in the culture water was between 102 and 105 CFU.mL-1. Five bacterial isolates were obtained from the water samples and periphytic culture on bamboo substrate samples. Based on the overall results, the use of periphytic biofiltration technology through the application of nitrification bacteria can potentially increase the stability of physical-chemical parameters of water quality and microflora community, supporting the growth and survival of prawn M. rosenbergii in nursery phase.