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Optimization of Physiochemical Parameters for Decolorization of Reactive Black HFGR Using Soil Fungus, Aspergillus allhabadii MTCC 9988

Rohilla SK, Salar RK and Kumar J

Aspergillus allhabadii MTCC 9988 isolated from pesticides sprayed cotton growing soil, were evaluated for decolorization of a textile dye, Reactive Black HFGR. The physiochemical parameters (shaking vs. static, pH, temperature, dye concentration and different carbon sources) were optimized and tested their effect on the degradation of textile dye in C-limited Czapek Dox media. Aspergillus allhabadii MTCC 9988 showed highest decolorization (90.57 ± 0.33%) of textile dye in shaking condition at temperature (30°C), pH (5.0), dye concentration (150 mg/L) and glucose as carbon source. Effect of different concentration of Reactive Black HFGR dyes ranging from 50 to 250 mg/L had a significant effect on decolorization (68.99 ± 0.67 to 90.57 ± 0.33%) with maximum decolorization at 150 mg/L and further increase in the concentration of dye showed a negative effect on the decolorization of the tested fungal strain. Different pH, ranging from 4 to 8 and temperatures ranging from 20-40°C also affected the decolorization efficiency of the tested fungal strain. However, optimum pH and temperature was found to be 5 and 30°C respectively. There was an influence of carbon source on decolorization, as the fungus showed considerable variation in decolorization activity in various carbon supplemented medium. The fungal strain showed maximum decolorization activity with glucose (90.57 ± 0.33%), followed by fructose (87.22 ± 0.22%) and sucrose (74.06 ± 0.55%). Therefore, bioaugmentation is also an excellent approach for dye degradation from the effluent and it might be a practical alternative in the dyeing wastewater treatment