बायोरेमेडिएशन और बायोडिग्रेडेशन जर्नल

अमूर्त

Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

Lamia Ayed, Nadia Chammam, Nedra Asses and Moktar Hamdi

Aspergillus niger has been tested in a discontinuous process for the pretreatment of fresh green Table Olive Processing Waste waters (TOPW). The influence of seven factors like incubation time, initial pH, dilution, glucose, agitation, (NH4)2SO4 and inoculum size on decolorization was screened by employing a fractional factorial design 27-4. Through factorial plots and analysis of variance, it was found that only glucose and agitation affected the decolorization. Further studies were carried out to adjust the level of determinant factors. Results showed that color removal (62%) was optimum at 150 rpm and 3 g/l glucose. HPLC and FTIR analysis of the treated TOPW suggested that the decolorization occurs through biosorption and biodegradation. Tannase and lignin peroxidase were detected in the cultures. However, only tannase is responsible for the color removal of the TOPW because lignin peroxidase is produced at a low level.