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Novel polysaccharide ? phenolic derivative of poly (glyceric acid ether) from different species of Boraginaceae family and its anticancer efficacy

Vakhtang Barbakadze

The Boraginaceae family comprises a group of plants that are important for medicine and pharmaceutics. The therapeutic effect of these plants is related to the content of many biologically active compounds. However, these plants are also rich in hepatotoxic pyrrolizidine alkaloids. The high molecular (>1000 kDa) water-soluble preparations from medicinal plants of Symphytum asperum, S.caucasicum, S.officinale, S.grandiflorum,  Anchusa italica, Cynoglossum officinale and Borago officinalis (Boraginaceae) were investigated. The fractionation of aforenamed preparations by means of ultrafiltration on membrane filter with cut off value of 1000 kDa permitted completely remove toxic pyrrolizidine alkaloids. Consequently the use of above mentioned plants does not rise any objection. The main chemical constit¬uent of high molecular preparations was found  to be poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene] or poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA)(1) (Fig. 1) according to data of liquid-state ¹H, ¹³C NMR, 2D ¹H/¹³C  HSQC, 2D DOSY and solid-state ¹³C NMR spectra. The polyoxyethylene chain is the backbone of this polymer molecule with a residue of 3-(3,4-dihydroxyphenyl)glyceric acid (2) (Fig. 2.) as the repeating unit. PDPGA as a phenolic derivative of poly(glyceric acid ether) belongs to a class of an acidic polysaccharides [poly(sugar acids)]. PDPGA exhibited anticomplementary, antioxidant, antiinflammatory, burn and wound healing and anticancer activities (Fig. 3). Human Hyaluronidase (Hyal-1) degrades high molecular Hyaluronic acid into smaller fragments which have pro-inflammatory effects. PDPGA possessed the ability to inhibit the enzymatic activity of Hyal-1 completely. Consequently PDPGA exhibited anti-inflammatory efficacy. Besides, PDPGA exerted anticancer activity in vitro and in vivo against androgen-dependent (LNCaP)  and –independent (22Rv1)   human prostate cancer (PCA) cells with comparatively lesser cytotoxicity towards non-neoplastic human prostate epithelial cells PWR-1E. PDPGA induced apoptotic death by activating caspases, and also strongly decreased androgen receptor and prostate specific antigen expression by 87%. Overall, this study identifies PDPGA as a potent agent against PCA without any toxicity, and supports its clinical application.