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अमूर्त

Isolation and Characterization of Octadecanoic Acid from The Ethyl Acetate Root Extract of Trigonella foneum graecum L. by Using Hydroponics Method

S Sudharsan, R Saravanan, A Shanmugam, S Vairamani, R Mohan kumar, S Menaga and N Ramesh

The octadecanoic acid (OA), methyl ester was isolated and characterized from root of fenugreek (T. foenumgraecum) using ethyl acetate (EAC) in hydroponics method. The OA was characterized by TLC & HPTLC and purified by HPLC. The structure and molecular weight (298) of OA was confirmed by GC-MS. The Rf value of sample (peak 4) was matched with standard. OA has strong anti-bacterial activity in B. subitlis and P. aeruginousa, where as weak activity in S. aureus and E. coli. Among the tested concentration of in vitro anti-oxidant activity of OA was maximum in 40µl and minimum in 20µl.