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Implementation of response surface methodology for phenol degradation using Pseudomonas putida (NCIM 2102)

V.Sridevi, M.V.V.Chandana Lakshmi, A.V.N. Swamy and M. Narasimha Rao

Phenol, a major pollutant in several industrial wastewaters is often used as a model compound for studies on biodegradation. Experiments were performed as a function of glucose (0.4-1.6 g/l), ammonium sulfate (0.5- 2.0 g/l) and concentration of metal ion, Mn2+ (0.01-0.04 g/l) by Pseudomonas putida (NCIM 2102), at constant phenol concentration 0.100 gL-1. Optimization of these three process parameters for phenol degradation was studied. Statistically designed experiments using response surface methodology (RSM) was used to get more information about the significant effects and the interactions between the three parameters. A 23 full - factorial central composite designed technique was employed for experimental design and analysis of the results. The optimum process conditions for maximizing phenol degradation (removal) were recognized as follows; glucose (0.8229 g/l), ammonium sulphate(1.5183 g/l) and metal ion concentration, [Mn2+.]( 0.0195 g/l) A maximum % phenol degradation of 98.24 was obtained at these optimum parameters.