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Endo Microbial Fauna of Tilapia spp. (Oreochromis niloticus) found in a Flowing Canal at Eden Garden and Park Utako, Abuja

Sheyin AN and Solomon KJ

A research conducted on the endofauna of fresh water Tilapia spp., (Oreochromis niloticus) found in a flowing canal or stream at Eden garden and Park, Opposite Chida hotel Utako Abuja, Nigeria. Sections of the skin, gills and intestine of twenty randomly selected fish were aseptically removed by means of sterile scalpel and pair of sterile scissors. Four (4 g) of each sections were homogenized in cml of sterile distilled water, which served as the original stock culture. A Serial dilution of 109 was carried out, and surface plated on nutrient agar. A total of eighteen (18) bacteria specie were isolated and identified. Twelve (12) bacteria were identified to the specie level and six (6) to the genus level. Eleven (11) were Gram positive namely. Bacillus spp.,, Staphylococcus spp.,, Streptococcus spp., Lactobacullus spp., Clostridium spp., Enterobacter aerogenes, Bacillus moratorium, Bacillus pumillus, Bacillus alvei, Bacillus licheniformis, Staphylococcus saprophyticus and Seven (7) Gram negative bacteria namely; Escherichia coli, Klebsiella pnemoniae, Pseudomonas alcaligenes Salmonella spp., Listeria monocytogenes, Serratia mercescens and providential stuertii. The frequency of occurrences of the isolated Bacteria indicated that Bacillus alvei had the highest frequency occurrence (12.72%), while Salmonella spp., had the least frequency occurrence (1.82%). The mean viable count from each sections of the samples revealed 50.1 × 107 Cfug-1 from the gills, 29.8 × 108 Cfug-1 from the isolates were found to be of medical importance to man.