हमारा समूह 1000 से अधिक वैज्ञानिक सोसायटी के सहयोग से हर साल संयुक्त राज्य अमेरिका, यूरोप और एशिया में 3000+ वैश्विक सम्मेलन श्रृंखला कार्यक्रम आयोजित करता है और 700+ ओपन एक्सेस जर्नल प्रकाशित करता है जिसमें 50000 से अधिक प्रतिष्ठित व्यक्तित्व, प्रतिष्ठित वैज्ञानिक संपादकीय बोर्ड के सदस्यों के रूप में शामिल होते हैं।
ओपन एक्सेस जर्नल्स को अधिक पाठक और उद्धरण मिल रहे हैं
700 जर्नल और 15,000,000 पाठक प्रत्येक जर्नल को 25,000+ पाठक मिल रहे हैं
S. P. Pawar, S. Patil, V. Chaudhari
Objective: The new, rapid, sensitive, simple, precise and accurate Reversed- Phase Ultra High Performance Liquid Chromatography (RP-UHPLC) method was developed and validated for determination of Sertraline in bulk drug and Pharmaceutical dosage form.
Methods: The UV Spectrum of Sertraline in diluent showed maximum wavelength at 273nm. In RP-UHPLC method separation achieved by Agilent C18 (75mm×3.9mm, 2μm particle size) column using Acetonitrile :( 0.1%OPA) Water (80:20v/v) as mobile phase at flow rate 0.7ml/min. Injection volume is 20μl. RP-UHPLC detection carried out at 273nm.
Results: In RP-UHPLC method retention time was found to be 3.75min. The Calibration curve was found to be linear (r2=0.999) with concentration range of 10-50μg/ml. The Accuracy (% recovery) for Sertraline was found to be 99-100%. The % RSD (intra-day and inter precision) values are not more than 2% hence the developed methods are accurate and precise. The LOD and LOQ were found to be 0.2085μg/ml and 0.6321μg/ml respectively.
Conclusion: The developed method was validated with respect to linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ as per ICH guidelines. The proposed method was used for routine analysis of Sertraline in Bulk drug and Solid dosage Form.